New specific primers for amplification of the Internal Transcribed Spacer region in Clitellata (Annelida)
نویسندگان
چکیده
منابع مشابه
New specific primers for amplification of the Internal Transcribed Spacer region in Clitellata (Annelida)
Nuclear molecular evidence, for example, the rapidly evolving Internal Transcribed Spacer region (ITS), integrated with maternally inherited (mitochondrial) COI barcodes, has provided new insights into the diversity of clitellate annelids. PCR amplification and sequencing of ITS, however, are often hampered by poor specificity of primers used. Therefore, new clitellate-specific primers for ampl...
متن کاملDetection and identification of mycobacteria by amplification of the internal transcribed spacer regions with genus- and species-specific PCR primers.
We evaluated the usefulness of PCR assays that target the internal transcribed spacer (ITS) region for identifying mycobacteria at the species level. The conservative and species-specific ITS sequences of 33 species of mycobacteria were analyzed in a multialignment analysis. One pair of panmycobacterial primers and seven pairs of mycobacterial species-specific primers were designed. All PCRs we...
متن کاملStudy of the Molecular Diversity of Internal Transcribed Spacer Region (ITS1.4) in Some Lettuce Genotypes
Lettuce is belong to the Cichorium family with leaf consumption, Annual cold weather plants and rich in minerals, vitamins and nutrients. The aim of the present study was to evaluate the genetic diversity of 15 varieties and lettuce populations using the ITS area. Sequence quality was evaluated using Chromas 2.1.1 software, aligned by MegAlign 5 software and then similarity matrix and dendrogra...
متن کاملThe internal transcribed spacer region of belonolaimus (nemata: belonolaimidae).
Belonolaimus isolates from six U.S. states were compared by restriction endonuclease digestion of amplified first internal transcribed spacer region (ITS1) of the nuclear ribosomal genes. Seven restriction enzymes were selected for evaluation based on restriction sites inferred from the nucleotide sequence of a South Carolina Belonolaimus isolate. Amplified product size from individuals of each...
متن کاملInternal transcribed spacer region evolution in Larix and Pseudotsuga (Pinaceae).
The nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) region has been characterized in the sister genera Larix and Pseudotsuga (Pinaceae). Complete sequences were obtained for seven species of Larix from North America and Eurasia and five species of Pseudotsuga from western North America and eastern Asia. ITS region lengths ranged from 1759 to 1770 bp in Larix and from 1564 to 157...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Ecology and Evolution
سال: 2017
ISSN: 2045-7758
DOI: 10.1002/ece3.3212